SEMPRE-BIO’s Final Event
SEMPRE-BIO’s Final Event Four Years Advancing Sustainable Biogas and Biomethane Solutions SEMPRE-BIO final event reports pilot findings and examines barriers to biomethane deployment Barcelona, 29 October – At its Final Event on 23 September, SEMPRE-BIO presented the results and limits of four years of work on biomethane production in Spain, France and Belgium. In the first roundtable, SEMPRE-BIO and three other European projects compared the setbacks they faced during demonstration and discussed the flexibility needed when plans changed. In the second, SEMPRE-BIO partners and external experts examined how regulation, market conditions and local acceptance affect wider biomethane use. SEMPRE-BIO is a Horizon Europe project testing different ways to produce biomethane from locally available resources. During the event’s first main session, its partners reported on three demonstration sites: At the Baix Llobregat wastewater treatment plant, SEMPRE-BIO aimed to produce biomethane for buses from biogas generated at the plant. The team advanced the development and validation of the proposed solution by testing key stages of biogas treatment and compression. In France, the team integrated pyrolysis, syngas cleaning and biological methanation and tested them with real syngas. The resulting biogas still needs upgrading before grid injection. At a dairy farm in Belgium, a cryogenic pilot separated methane-rich gas and a high-purity CO₂ stream from farm biogas. Testing identified further work needed to reduce energy use and improve equipment design. The project also investigated uses for CO₂ and digestate, the nutrient-rich material left after anaerobic digestion. Experiments included production of succinic acid, PHA biopolymers and protein-rich biomass. Across the different case studies, the project also used modelling to assess costs, greenhouse gas emissions and broader social impacts associated with the scale-up of the proposed solutions. In the first roundtable, moderated by Alejandra Córdova, SEMPRE-BIO Project Coordinator, representatives of Biomethaverse, HyFuelUp, Value4Farm shared experiences and lessons learned from the demonstration phase of their respective projects. The discussion highlighted challenges related to equipment sizing and availability, changes in demonstration sites, financing, technology integration and safety requirements such as ATEX. These experiences underlined the importance of flexibility, rapid adaptation and early identification of alternative pathways when developing and validating innovative technologies, helping projects respond effectively to unforeseen challenges and maximise the results achieved. The second roundtable, with Mario Ruiz, Head of Climate and Environmental Action at Aigües de Barcelona, as moderator, addressed barriers that remain even when a process can be operated. Participants from the European Biogas Association, Biogas Danmark, Naturgy, Veolia, TMB and BETA Technological Centre discussed permitting, access to gas networks, certification and guarantees of origin, limitations to cross-border trade, the lack of sufficient incentives, market development for biogas/biomethane and co-products, and local acceptance. Bruno Sander Nielsen of Biogas Danmark pointed to Denmark’s 2012 premium for upgrading biogas and access to the gas grid on reasonable terms at the time. Together, he said, they helped projects that had been planned for years secure financing and get built. Asked to name one change that could speed deployment, Pablo Molina of the European Biogas Association argued that there was no single fix: incentives to produce or buy biomethane would have limited effect if permitting, grid connection and delays in implementing policy remained unresolved. The event concluded with a visit to the SEMPRE-BIO demonstration site at the Baix Llobregat Wastewater Treatment Plant in El Prat de Llobregat (Spain), operated by Aigües de Barcelona. Participants had the opportunity to see first-hand the biological methanation and hydrogen production systems developed as part of the project, as well as discuss operational experiences, technical challenges and lessons learned directly with the project partners responsible for the pilot. SEMPRE-BIO has generated knowledge about the opportunities and technical challenges of three different biomethane pathways, including what their integration at operating sites requires and where further development is needed. The Final Event placed those findings alongside the experience of other projects and the regulatory and market conditions that will influence wider deployment in Europe. About SEMPRE-BIO SEMPRE-BIO, SEcuring doMestic PRoduction of cost-Effective BIOmethane, is a Horizon Europe project involving 17 partners. Media contactLaia MenciaInveniam Group – Project Managerlaia@inveniam-group.com+34 930 181 691 EU funding acknowledgement and disclaimer Funded by the European Union under Grant Agreement No. 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Climate, Infrastructure and Environment Executive Agency (CINEA). Neither the European Union nor CINEA can be held responsible for them. Author: Laia Mencia Editorial: Lucía Salinas Date: October, 2026 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
Join SEMPRE-BIO’s Final Event
Join SEMPRE-BIO’s Final Event Four Years Advancing Sustainable Biogas and Biomethane Solutions Final event After four years of research, innovation, and collaboration across Europe, the SEMPRE-BIO project is reaching the end of its journey. During this time, the project has contributed to advancing sustainable biogas and biomethane production pathways, demonstrating innovative technologies, and supporting the transition towards a more circular and climate-neutral economy. To celebrate the project’s achievements and share its key results, we are pleased to invite you to SEMPRE-BIO’s Final Event, where project partners, industry experts, policymakers, and stakeholders will come together to discuss lessons learned, future opportunities, and the role of biogas and biomethane in Europe’s energy transition. Date and Location Location: Edificio D38 (Sede Veolia), Paseo de la Zona Franca, 48 (08038 – Barcelona) Date: 23 September 2026 Time: 09:00 CET Registration Mark your calendar and secure your place today: Register here: Final Event: Rellenar formulario Participants may choose to attend either in person or online. Online attendees will receive an email with the access link the day before the event. In-person attendees will receive a reminder email during the week prior to the event. Please remember to check your email for the latest event information. Event Agenda The morning starts with what came out of the project. Over the past years we have produced biomethane from three very different wastes: the biogas from a wastewater treatment plant near Barcelona, woody prunings and branches near Bourges, and biogas from a dairy farm in West Flanders. Each site followed its own route, and a fourth strand of work looked at what to do with the CO₂ that every upgrading process leaves behind. The people who did the work will present what they obtained at each site. The rest of the day looks outwards. The first roundtable puts several Horizon Europe biomethane projects in the same room to talk about the obstacles they ran into while building their demonstration plants. The second follows the value chain, from the operators whose plants produce the feedstock through to the gas utility that injects and sells the biomethane and the transport operator whose buses already run on it, to discuss what would need to change for biomethane to be produced at a larger scale. For those attending in person, the afternoon ends at one of the pilot sites, where the technology described during the morning can be seen running. The full programme is available below: We look forward to welcoming you to Barcelona and celebrating the achievements of the SEMPRE-BIO project together as we reflect on four years of collaboration and explore the future of sustainable biomethane in Europe. Author: Laia Mencia Editorial: Lucía Salinas Date: August, 2026 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
CO2 Valorization Technologies
CO₂ Valorization Technologies Case Study 3 progress update on bioproduct recovery from CO₂ and digestate at pilot scale Quick recap In this blog, we provide a concise overview of the progress made toward the commissioning of CS3, one of the three European Biomethane Innovation Ecosystems (EBIEs) developed within the SEMPRE-BIO consortium. Regarding CO₂ valorization, we tested two technologies: Hybrid fermentation to produce valuable biochemicals (succinic acid) and biopolymers. Microalgae and purple phototrophic bacteria (PPB) for alternative protein production. These biotechnologies aim to recover the waste streams from the anaerobic digestion process of CS-III, i.e., CO₂ from biogas upgrading and the liquid fraction of digestate. Key Milestones Achieved Several important milestones have now been reached: Equipment installation and start-up Both CO₂ valorization technologies have been implemented at pilot scale (TRL-7). A 50-L hybrid fermenter coupled to an intensive mass transfer unit (IMTU) has been designed and installed at UVIC’s facilities to produce succinic acid and biopolymers from CO₂. Regarding microalgae, a 600-L solar photobioreactor (sPBR) has been built for outdoor microalgae cultivation at UVIC, whereas INNOLAB has installed a 1250-L conventional photobioreactor (cPBR) to cultivate green microalgae inside a greenhouse (under artificial illumination). At UVIC, a 100-L vertical PBR has also been installed for the cultivation of PPB under anaerobic conditions. Lab scale processes optimization UVIC has studied the fermentation process at lab-scale for biopolymers and organic acids production. These results have been used for upscaling the process (under evaluation). UVIC has also studied the cultivation of PPB at lab-scale, obtaining promising results. Preliminary results on the pilot-scale operation Through research and experimentation, UVIC and INNOLAB fine-tuned their cultivation methodologies to ensure maximum photosynthetic efficiency when growing in synthetic medium. The use of liquid fraction of digestate and CO₂ from a local biogas facility is under evaluation. Analysis of the amino acid profile of microalgae A study has been conducted to ensure that the quality of the cultivated protein is adequate for feeding applications. The biomass from different strains has been tested under both synthetic growth media and digestate conditions. Evaluation of the digestibility potential of microalgae biomass The biomass produced in SEMPRE-BIO is being evaluated by simulating the digestibility of their biomass by farm animals. Next Steps The next steps of CO₂ valorization technologies focus on optimising the biological processes: For the hybrid fermenter, improve the gas-liquid transfer to increase the productivity of succinic acid from CO₂, and liquid fraction of digestate (UVIC) Improve the production of microalgae and PPB biomass grown in the liquid fraction of digestate and recovered CO₂ (UVIC, INNOLAB) Increase scientific knowledge of the photosynthetic performance of green microalgae under the growing conditions of SEMPRE-BIO project. Further optimise the processes Complete the analysis of amino acid profiles and digestibility potential of microalgae and PPB biomass. Long-term Vision SEMPRE-BIO’s mission is to increase the feasibility and sustainability of anaerobic digestion systems. Anaerobic digestion is a well-established technology with high production potential in Europe, playing a key role in the decarbonisation of the energy sector. However, dealing with its sub-products (residual CO₂ and digestate) is challenging and implies limited deployment of this type of facilities. Hybrid fermentation or cultivating microalgae/PPB using CO₂ as carbon source and digestate as source of nutrients is a green and sustainable solution to close the nutrient and carbon loops of the digestion process, obtaining added-value bio-product (alternative protein and succinic acid) as by-product, thus minimising socio-economic and environmental impacts. This solution transforms conventional anaerobic digestion plants into novel biorefinery units where different conversion processes are integrated to obtain multiple bioproducts, with the goal of maximising profitability and reducing waste. This solution opens new business opportunities by closing the loop in the agri-food sector, obtaining energy and feed sources from agri-food wastes. Preliminary results are promising, and work is ongoing on the optimisation of the production processes. The main barriers to developing a business plan are legislative and social. We are working on developing risk management plans to ensure that the production of alternative protein is safe, as well as on integrating this technology into innovative certification schemes. These instruments can increase the market potential of the solution by providing reliable and transparent information on the characteristics and origin of the feedstocks and bio-products, with the goal of increasing consumer acceptance. For more information, check the CO₂ valorization video here: https://www.youtube.com/watch?v=Zcj-xKdEXWc Author: Josué González Editorial: Lucía Salinas and Laia Mencia Date: May, 2026 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
CS-3 Demo Plant Update
CS-3 Demo Plant Update From the lab to the field: cryogenic technology applied to a real-world setting Quick recap This article provides an update on the current status of CS3, one of the three European Biomethane Innovation Ecosystems (EBIEs) within the SEMPRE-BIO consortium. CS3 takes place at the Masscheleyn dairy farm in Adinkerke, West Flanders (Belgium), where an anaerobic digestion plant processes cattle manure and co-substrates, producing over 110 m³ of biogas per hour. Partners CryoInox, Innolab and Ghent University have collaborated on the implementation of a cryogenic upgrading demo plant that converts raw biogas into two products: bio-LNG and liquid CO₂. For a full description of CS3, the best starting point is the original case study article on the SEMPRE-BIO website. Key milestones Achieved Several important milestones have now been reached: Demo plant installation and start-up: The cryogenic upgrading plant was delivered, installed and started up as a complete system in a real operational environment. The key functions of the cryogenic process have been successfully tested. First industrial-grade CO₂ batch produced and bottled: In July 2025, the first batch of industrial-grade CO₂ was produced and analysed by a certified laboratory. This is a concrete milestone that confirms the plant’s ability to separate CO₂ from raw biogas through an integrated cleaning process. Resolution of initial operational issues: Leakages were fixed, compressors maintained, refrigerant added, and control valves replaced. The system is currently operating correctly in a real-world context. Next steps CS3 has now entered its optimisation and validation phase. From January 2026, the team has been systematically applying the optimisations identified in the preceding months, with the goal of improving the quality of the produced molecules and bringing the plant toward stable, monitorable operation over a sufficiently long period. This phase is aimed at collecting reliable and structured data for the validation of the project KPIs: production cost per Nm³ of biomethane, utilities consumption, and a minimum processing capacity of 50 Nm³/h. In parallel, certified laboratory analyses are being prepared for the formal validation of the quality of the biomethane and CO₂ produced. Long-term vision CS3 aims to bring cryoseparation, appropriately downsized, to small-scale agricultural applications, a segment often excluded from traditional industrial solutions. The province of West Flanders has approximately 8,000 farms: a compact and replicable system like the one demonstrated in Adinkerke could open a concrete pathway for valorising agricultural biogas in regions with high livestock density. The approach is circular: digestate becomes fertiliser in France, liquid CO₂ feeds microalgae cultivation in Belgium, and bio-LNG can serve as fuel for the farm’s own agricultural vehicles. The data collected will feed into the final report D3.3, contributing to the techno-economic foundation for the broader deployment of this technology in the European biomethane market. For more information, check the Case Study 3 video here: https://www.youtube.com/watch?v=QxP5hqOtw2c Author: Andrea Munaretto Editorial: Lucía Salinas and Laia Mencia Date: March, 2026 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
CS-2 Pilot Plant Update
CS-2 Pilot Plant Update Progress toward commissioning a biomethane pilot plant A Quick recap In this blog page, we provide a concise overview of the progress made toward the commissioning of CS2, one of the three European Biomethane Innovation Ecosystems (EBIEs) developed within the SEMPRE-BIO consortium. At our partner TerraWatt’s site in Marmagne (France), we aim to valorize green waste and agroforestry residues by developing an innovative process that combines pyrolysis and biomethanation. The first step of the process is biomass pyrolysis, a thermochemical conversion that produces two valuable outputs: Biochar, a stable carbon-rich material that acts as a long-term carbon sink, and Syngas, a gaseous mixture composed of several compounds. In the second step, this syngas undergoes biomethanation. In this biological process, specialized microbial communities convert the raw (“dirty”) syngas into biogas, mainly composed of biomethane and CO₂. Key milestones Achieved Several important milestones have now been reached: Equipment installation: The pyrolysis unit has been installed, and the biomethanation unit has been constructed in line with the project design. A dedicated pipeline connecting the two units is now in place, enabling the transfer of syngas to the biomethanation reactors. All auxiliary systems, such as heating and cooling circuits, compressed air, and utilities, have also been installed. Equipment integration: Software installation, input/output checks, and leak tests have been successfully completed. These steps confirmed that all sensors, control systems, and equipment are properly connected and fully operational. Safety assessment: A comprehensive safety study has been conducted to ensure that all necessary preventive and protective measures are implemented. Pyrolysis commissioning: The pyrolysis unit has been tested using several types of biomass. Initial start-up challenges were identified and resolved, allowing the unit to operate reliably. Next steps The next major milestone is the commissioning of the fully integrated system, coupling the pyrolysis and biomethanation units. Because biomethanation is a biological process, the reactors must first be inoculated with the appropriate microbial communities. The operating conditions, particularly temperature, will be carefully controlled to allow the bacteria to grow, while syngas is progressively supplied. Once a stable biological activity is established, we will begin testing different operating conditions to identify the parameters that deliver the highest syngas-to-biogas conversion efficiency. Long-term vision TerraWatt’s mission is to support climate change mitigation by producing renewable biomethane and biochar, a durable carbon removal solution. Their ambition is to deploy multiple plants in regions where biomass is locally available and currently underutilized. This integrated approach addresses climate goals through both renewable energy production and long-term carbon storage, creating value from resources that would otherwise go unused and supporting circular, low-carbon business models. For more information, check the Case Study 2 video here: https://www.youtube.com/watch?v=Bnt0btre54g Author:Paolo de Carli Editorial: Lucía Salinas and Laia Mencia Date: January, 2026 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
SEMPRE-BIO 6th General Assembly in Ghent
SEMPRE-BIO 6th General Assembly in Ghent A site-by-site account of technical progress The SEMPRE-BIO consortium met in Ghent on 13–14 November 2025 for its sixth General Assembly. Hosted by Ghent University, the meeting provided a space to review progress, explore technical issues and outline upcoming steps towards domestic, cost-effective biomethane production. Over two days of presentations, discussions and site visits, partners reiterated their commitment to advancing biomethane, bioproducts and circular bioeconomy pathways. Where each site stands Case Study 1: Biomethanation and Electrolysis (Cetaqua) Case Study 1 reported solid progress despite setbacks related to the commissioning of the PEM electrolyser and biomethanation unit. Repairs following leakages, follow-up from the ATEX assessment and ongoing inoculum adaptation tests presented a realistic assessment of both challenges and promising developments. Key next steps include completing I/O checks, ensuring UPW supply and moving towards final commissioning. Case Study 2: Pyrolysis–Biomethanation Integration (TerraWatt) TerraWatt reported progress on both the pyrolysis and biomethanation systems. Early-stage issues related to cabling, automation logic, kiln performance and the heat exchanger’s integrity were detected and are currently being addressed. The biomethanation unit has already been installed and is now undergoing automation set-up and data-logging configuration. Case Study 3: Anaerobic Digestion and Cryogenic Upgrading (CryoInox & UGent) Case Study 3 reported significant achievements. The anaerobic digestion system at the farm continues stable operation, and the cryogenic upgrading demo plant has produced its first industrial-grade CO2 batch, marking a key step towards validating cryoseparation technology. Optimisation remains central, with continuous operation targeted for early 2026. Parallel research on biogas desulfurisation using sulfide-oxidising bacteria also showed encouraging progress. CO2 valorisation UVic presented progress on succinic acid, PHA and microbial biomass production. Pressurised fermentation conditions are improving succinic acid yields, while microalgae and purple phototrophic bacteria grown on digestate-based media are delivering protein-rich outputs. These advances move the project closer to integrated bioproduct valorisation strategies. Work with biogenic CO2 will continue refining these processes. The sustainability and modelling teams from DBFZ and SINTEF shared updates on techno-economic, environmental and social assessments. Their findings confirmed that biomethane can be cost-covering at relatively low electricity prices, and environmental modelling revealed impressive mitigation potentials across the three case studies—particularly in systems benefiting from manure management efficiencies and biochar carbon sequestration. Dissemination, communication and exploitation activities were also reviewed. The project continues to reach broad audiences with strong engagement metrics. With Phase I of the tech-to-market plan nearing completion, work will soon shift towards more advanced exploitation actions and preparation of the project’s business plan. The management session confirmed that financial and administrative work is progressing as planned and partners also discussed the schedule for upcoming assemblies: an online meeting followed by the final event and General Assembly in the next year. Field Visit The second day was dedicated to site visits. Partners visited the dairy farm De Zwanebloem with integrated anaerobic digestion, the cryogenic upgrading demo plant from Case Study 3 and the Innolab facilities with a photobioreactor for microalgae cultivation. These visits allowed partners to observe operational systems first-hand and appreciate the technical complexity behind biomethane production, CO2 valorisation and microbial bioprocessing. What’s next This Ghent assembly highlighted the substantial progress achieved and the increasing interconnection between work packages and case studies. As the project enters its next phase, priorities include finalising commissioning, addressing remaining technical issues, deepening environmental and economic modelling, and strengthening communication and exploitation activities to ensure lasting impact beyond the research community. Author: Oria Pardo Editor: Laia Mencia & Lucía Salinas Date: November, 2025 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
CS-1 Pilot Plant Update
CS-1 Pilot Plant Update Moving from design to reality Quick recap This blog focuses on Case Study 1 and the progress made so far. Case Study 1 started in 2023 and has evolved greatly since then. It is one of our three EBIEs (European Biomethane Innovation Ecosystems), which are experimental sites where we develop and test new technologies. Here, partners CETAQUA, DTU, SINTEF, BIOTHANE and PROPULS are producing biomethane using wastewater at El Baix Llobregat WWTP. The aim is to use this green fuel to power two TMB buses in Barcelona’s public transport network. If you want to understand it from scratch, this is where to look. For those interested in where we stand three years in, this blog outlines the progress in bringing our CS-1 pilot plant to life. What started as detailed engineering drawings has now become a fully constructed facility ready for operation. Key milestones Here are the key achievements we’ve reached: Site Preparation & Infrastructure: We adapted a section of the wastewater treatment plant (WWTP), including soil studies and concrete slab installation, to safely house our containerized PEM electrolyzer (used to split water into hydrogen and oxygen). Equipment Installation: The PEMEL was delivered in February 2025. The biogas purification skid, used to remove impurities from the biogas, followed in March, and the biomethanation reactor in May. All major equipment is now in place and connected. Utilities Integration: We completed all tie-ins for water, electricity and future biogas supply, coordinating carefully with WWTP operations to minimize disruption. Commissioning Phase: Software installation, I/O checks, and leak testing have been completed, confirming all sensors and equipment are properly connected and functional. Next steps After completing construction, installation and initial commissioning, we’re now ready to begin plant operation. This starts with inoculation, which is the introduction of the microorganisms that will drive the biomethanation process into the reactor. The following stage involves low-flow experiments, where feedstock is added slowly to allow the process to stabilize. Flows will be gradually increased until we reach design productivity for biomethane production. Long-term vision Our ultimate goal is to produce locally generated green biomethane that will power TMB buses, creating a sustainable circular economy solution that transforms wastewater treatment byproducts into clean transportation fuel. CS-1 is also an important step in validating how biomethane can be produced and integrated within existing public infrastructure. The experience gained here could guide future projects, helping establish reliable models for the broader deployment of green biomethane in Europe. For more information, check the Case Study 1 video here: https://www.youtube.com/watch?v=ntWCdUZoPaA Author: David Checa Editorial: Lucía Salinas and Laia Mencia Date: October, 2025 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
Life cycle assessment
Life cycle assessment LCA methodology and comparison with fossil fuels Introduction The importance of sustainability and environmental impact is currently increasing, and methods to identify and classify these characteristics are becoming more prevalent as a result. One such method is Life-Cycle Assessment. What is an LCA? Life-Cycle Assessment, or LCA, is a powerful method of examining a product’s entire environmental impact, from the extraction of raw materials to its disposal or recycling. Often referred to as a ‘cradle-to-grave’ analysis, LCA enables us to see how our products affect the planet throughout their entire lifespan. Whether you are looking at a physical product or the service it provides, LCA breaks things down into three essential steps: Measuring what goes in and comes out: This includes tracking energy use, raw material consumption, emissions and waste. Evaluating the impact: What kind of environmental footprint do those inputs and outputs leave behind? Exploring better alternatives: How can we reduce harm and make smarter, more sustainable choices? LCAs help businesses and individuals to boost resource efficiency, cut environmental liabilities and move closer to truly sustainable design and decision-making. [1] How is it done? LCA is defined by ISO 14040. To execute an LCA tools such as the ILCD Handbook and the Environmental Footprint methods have been developed to support its practical application, especially in the EU. The LCA process is divided into four main phases. The first phase involves defining the goal and scope. This phase establishes the purpose, audience, functional unit and system boundaries. Next is the Life Cycle Inventory phase, which involves collecting data on energy, materials, emissions and waste inputs and outputs throughout the product system. Once the inventory is complete, the Life Cycle Impact Assessment begins, linking the data collected in the inventory to environmental impact categories using standardised models. This phase includes four sub-steps: Classification: inventory inputs and outputs are assigned to relevant impact categories. Characterisation: their contribution to each category is quantified using substance-specific factors. Normalisation: the results are scaled relative to a reference unit to show the significance of impacts. Weighting: impact categories are ranked by importance and an overall environmental score is generated. While normalisation and weighting are optional under ISO 14040, they are mandatory in the EU’s Environmental Footprint methods. Finally, the interpretation analyses the results for consistency and reliability, aligning them with the original study objectives. [2] Comparison between a fossil fuel and SEMPRE BIO’s LCA Using the LCA methodology to compare gasoline and SEMPRE-BIO’s biomethane reveals significant differences in environmental performance across key impact categories. The life cycle of gasoline is characterised by a high global warming potential (GWP), primarily due to direct CO₂ emissions during combustion and the extraction and refining of fossil fuels. Its environmental profile also includes elevated impacts related to fossil resource depletion and human toxicity, and there are no mechanisms in place to offset emissions. In contrast, the LCA of SEMPRE-BIO’s biomethane, which is produced from waste biomass and upgraded using renewable hydrogen, demonstrates markedly lower lifecycle emissions. With over 80% CO₂ conversion efficiency, SEMPRE-BIO minimises GWP and actively reduces net emissions through CO₂ valorisation. This process converts captured carbon into valuable products such as biopolymers and proteins, which improves its environmental footprint further. Compared with other biofuels such as bioethanol, which despite having a lower GWP can contribute to acidification and eutrophication due to fertiliser-intensive feedstocks, the use of waste streams and closed nutrient loops in SEMPRE-BIO‘s production process is advantageous. By mitigating these environmental trade-offs and aligning with the EU’s Environmental Footprint methodology, where normalisation and weighting enhance comparability, SEMPRE-BIO offers a comprehensive and robust sustainability profile. Ultimately, by integrating circular economy principles and aligning with EU climate and energy objectives, SEMPRE-BIO is positioned as a forward-looking model for decarbonised transport solutions. [3] Fonts: [1] https://www.eea.europa.eu/help/glossary/eea-glossary/life-cycle-assessment [2] https://eplca.jrc.ec.europa.eu/lifecycleassessment.html [3] https://re.public.polimi.it/retrieve/e0c31c12-6bf8-4599-e0531705fe0aef77/ Nobili_2020_Comparative LCA.pdf Author: Sofiia Savchenko Editorial: Lucía Salinas and Laia Mencia Date: July, 2025 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
European Cities Leading the Biomethane Public Transport
European Cities Leading the Biomethane Public Transport From Pamplona to Tallinn, how are municipalities decarbonizing public transport? Introduction Municipalities are actively seeking cleaner alternatives to decarbonize the way we move around. In this context, biomethane has emerged as one of the most promising solutions, as it causes fewer CO₂ emissions than petrol. Moreover, since biomethane is a purified version of biogas, its higher calorific value translates into greater efficiency when powering engines. That’s why, in this blog, we want to highlight successful case studies across Europe—soon to be joined by two new biomethane-powered buses that will circulate through the streets of Barcelona, thanks to SEMPRE-BIO and its Case Study 1. Paris, France The public transport company RATP has committed to converting part of its diesel fleet to BIOGNV (renewable natural gas for vehicles). So far, it has adapted 13 of its bus depots to operate with biomethane, and by 2025, it plans for 25% of its buses to run on biomethane—significantly reducing CO₂ emissions and local pollution. Moreover, part of the biomethane used is sourced from organic waste collected in the Île-de-France region, effectively closing the loop locally. Nottingham, United Kingdom Nottingham City Transport operates the world’s largest fleet of double-decker buses powered by biomethane. By 2022, the fleet had grown to 143 Enviro400 CBG vehicles, acquired in several phases since 2017. Sweden Linköping This Swedish city began transitioning its fleet in the 1990s and is now one of the first in the world to operate a bus network entirely powered by biogas. Today, all urban buses in Linköping run on biomethane produced from agricultural waste and sewage sludge. The success of this model has also led to its adoption in taxis, trucks, and municipal vehicles. Malmö Malmö uses biomethane produced from urban organic waste, including leftovers from supermarkets and households. Its biomethane-powered bus fleet has significantly reduced emissions from the transport sector. Additionally, the digestate from the process is returned to the fields as fertilizer, promoting a circular agri-food model. Stockholm Stockholm has been a pioneer in adopting biomethane-powered buses as part of the Baltic Biogas Bus project. This initiative, led by Stockholm’s public transport authority, aims to increase the use of biogas in urban transport across the Baltic region. Pamplona, Spain In 2022, Pamplona became the first city in Spain to operate a 100% renewable gas-powered urban fleet. The biomethane used is produced from sewage sludge and organic waste, and it powers more than 140 buses. This results in a 90% reduction in emissions compared to diesel. Bologna, Italy In Bologna, four urban buses and twenty taxis run on locally produced biomethane derived from organic and pruning waste. The project involves the public transport company TPER and the taxi operator CO.TA.BO. Tallinn, Estonia Tallinn has introduced new buses powered by biomethane and plans to continue using this fuel beyond 2030, even as other EU countries shift toward electric buses. It is worth highlighting the case of Sweden, where since 2008 the Public Transport Agreement Committee has been working toward a shared vision of sustainable mobility. Today, the country’s public coach fleet is almost entirely fossil fuel-free. Anna Grönlund, Deputy Director of the Swedish Bus and Coach Federation, attributes this success to the ability to align public procurement requirements with their own standards and recommendations for the tendering process. At SEMPRE-BIO, we believe that examples like Sweden’s can help pave the way and contribute their successes to a more sustainable global mobility future. Fonts: Biomethane in the Paris region to power RATP buses | natrangroupe.com Nottingham City Transport – Wikipedia Biogas – the natural choice for city buses Green project in Bologna: 4 city buses – Sustainable Bus Estonia wants to continue using biomethane in buses after 2030 | News | ERR Biokaasu & GoO ET:n kaasumarkkinatoimikunnan tapaaminen 2.12.2019 Susanna Pflüger Sweden’s buses are now nearly 100% fossil-free | IRU | World Road Transport Organisation Moventis TCC Pamplona adquiere 13 buses Scania propulsados por biometano | Scania España Author: Oria Pardo Editorial: Lucía Salinas and Laia Mencia Date: June, 2025 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
SEMPRE-BIO’s 5th General Assembly
SEMPRE-BIO’s 5th General Assembly An up-to-date overview from Spring 2025 The SEMPRE-BIO consortium met online for our 5th General Assembly on Thursday, May 22. This online meeting sought to check our progress and better coordinate the future of our work on turning residue into biomethane. Since our last meeting in Denmark back November 2024, quite a bit has happened. So where are we on moving from building to producing? The simple answer is we’re almost there but still need a few steps. We’ve worked through some problems with permit delays, equipment supply issues and construction hiccups. But what matters is we’ve worked through these matters and every pilot plant will be operational by July. Keep reading to understand what’s going on in each front. Where each site stands CS1 is still in build mode. They’ve redesigned the plant for better performance and started construction, but they’re waiting for final components to arrive. CS2 has made strong progress. After redesigning their reactors to improve flow and simplify the process, they’ve installed the pyrolysis equipment and plan to start operations in June. Now, they’re moving on to commissioning the biomethanation component and a few final installations to bring everything together by July. CS3 is the furthest along. Their plant is fully built and they’ve already completed their first tests using pure methane. They’re now transitioning to actual biomethane production. Next steps include verifying all production stages, calibrating the system, checking the quality of both the biomethane and captured CO2, and wrapping up with team training once testing is complete. Beyond plants While the pilot plants grab most attention, our CO2 valorisation work is quietly making progress: Biobased materials from CO2: The team has scaled up fermentation systems that convert CO2 and hydrogen into useful biochemicals and biopolymers. Early tests led to a lab-scale System that now produces PHA – a biodegradable plastic – from the bacteria Cupriavidus necator. CO2 to protein: We’re exploring how photosynthetic organisms can turn captured CO2 into alternative proteins. Outdoor trials with Parachlorella kessleri, a green microalga, have shown strong results, particularly in essential amino acid content. Early lab tests are also underway with purple bacteria, adding another potential pathway for turning captured CO2 into valuable biomass. What’s next Our eyes are set on July. That’s when construction ends and production begins across all sites. After months of planning and building, we’ll finally see residue becoming clean biomethane through our pilot operations, and captured CO2 used to produce bioplastics and proteins. Author: Lucía Salinas Date: May, 2025 This project has received funding from the European Union’s HORIZON-CL5-2021-D3-03-16 program under grant agreement No 101084297. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them. Follow us Facebook Twitter Linkedin Contact us info@sempre-bio.com Cookies Policy Privacy policy ©2023 Semprebio | All Rights Reserved | Powered by Scienseed
